Verified — all release criteria met
released
Certificate of analysis
GHK-Cu
Lot CP-GHKCU-2605B · 100 mg
99.46 %
HPLC purity
Spec ≥ 99.0 %
83.3 %
Net peptide content
By amino acid analysis
< 0.60
Endotoxin (EU/mg)
Spec < 5.0 EU/mg
Provenance
- Testing laboratory
- Colmaric Analyticals
- Accreditation
- ISO/IEC 17025:2017
- Country of synthesis
- United States
- Manufactured
- May 3, 2026
- Release tested
- May 12, 2026 (5 months ago)
- Retest date
- May 2, 2028
- CAS number
- 89030-95-5
- Molecular formula
- C14H24N6O4Cu
- Theoretical mass
- 403.94 Da
- Observed mass (LC-MS)
- 404.07 Da (Δ 0.13 Da)
Assay results
Every line the release panel ran for this lot, with the method, the acceptance criterion and the measured value — not a pass mark alone.
| Method | Parameter | Specification | Result | |
|---|---|---|---|---|
| RP-HPLC (UV 214 nm) | Purity (area %) | ≥ 99.0 % | 99.46 % | Pass |
| LC-MS (ESI+) | Identity — observed mass | 403.94 ± 1.0 Da | 404.07 Da | Pass |
| Amino acid analysis | Net peptide content | ≥ 80.0 % | 83.3 % | Pass |
| Karl Fischer | Water content | ≤ 8.0 % | 1.52 % | Pass |
| Ion chromatography | Residual TFA | ≤ 1.0 % | 0.33 % | Pass |
| LAL kinetic chromogenic | Bacterial endotoxin | < 5.0 EU/mg | < 0.60 EU/mg | Pass |
| USP <71> membrane filtration | Sterility | No growth | No growth | Pass |
| Visual / USP <790> | Appearance | White to off-white cake, free of visible particulates | Conforms | Pass |
| ICP-MS | Copper content (molar ratio Cu:peptide) | 0.95 – 1.05 : 1 | 1.002:1 | Pass |
| ICP-MS | Free (uncomplexed) copper | ≤ 0.5 % | 0.10 % | Pass |
Sequence
H-Gly-His-Lys-OH · Cu(II)
Handling for this lot
- Appearance
- Deep blue lyophilised powder — colour is the copper complex and is expected
- Storage
- -20 °C lyophilised, protected from light. Copper complexes are photosensitive.
- Reconstitution
- Use non-chelating diluent. EDTA or citrate buffers will strip the copper and leave you with the free tripeptide.
Other lots of GHK-Cu
Compare batch to batch. Consistency across lots is a stronger signal than any single certificate.
Research use only. This certificate describes material supplied strictly for laboratory research. It is not a drug, food, cosmetic or medical device, has not been evaluated by the FDA, and is not intended for human or veterinary administration of any kind.