Certificate of analysis
LL-37
Lot CP-LL37-2605B · 10 mg
99.16 %
HPLC purity
Spec ≥ 95.0 %
83.2 %
Net peptide content
By amino acid analysis
< 0.10
Endotoxin (EU/mg)
Spec < 5.0 EU/mg
Provenance
- Testing laboratory
- Janoshik Analytical
- Accreditation
- ISO/IEC 17025:2017
- Country of synthesis
- United States
- Manufactured
- May 6, 2026
- Release tested
- May 15, 2026 (4 months ago)
- Retest date
- May 5, 2028
- CAS number
- 154947-66-7
- Molecular formula
- C205H340N60O53
- Theoretical mass
- 4493.30 Da
- Observed mass (LC-MS)
- 4493.22 Da (Δ 0.08 Da)
Assay results
Every line the release panel ran for this lot, with the method, the acceptance criterion and the measured value — not a pass mark alone.
| Method | Parameter | Specification | Result | |
|---|---|---|---|---|
| RP-HPLC (UV 214 nm) | Purity (area %) | ≥ 95.0 % | 99.16 % | Pass |
| LC-MS (ESI+) | Identity — observed mass | 4493.30 ± 1.0 Da | 4493.22 Da | Pass |
| Amino acid analysis | Net peptide content | ≥ 80.0 % | 83.2 % | Pass |
| Karl Fischer | Water content | ≤ 8.0 % | 4.68 % | Pass |
| Ion chromatography | Residual TFA | ≤ 1.0 % | 0.06 % | Pass |
| LAL kinetic chromogenic | Bacterial endotoxin | < 5.0 EU/mg | < 0.10 EU/mg | Pass |
| USP <71> membrane filtration | Sterility | No growth | No growth | Pass |
| Visual / USP <790> | Appearance | White to off-white cake, free of visible particulates | Conforms | Pass |
Sequence
H-Leu-Leu-Gly-Asp-Phe-Phe-Arg-Lys-Ser-Lys-Glu-Lys-Ile-Gly-Lys-Glu-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe-Leu-Arg-Asn-Leu-Val-Pro-Arg-Thr-Glu-Ser-OH
Handling for this lot
- Appearance
- White lyophilised powder
- Storage
- -20 °C lyophilised. Aliquot before freezing — this peptide tolerates freeze-thaw poorly.
- Reconstitution
- Use low-protein-binding tubes and pipette tips. Adsorptive loss to standard polypropylene is significant at working concentrations.
Other lots of LL-37
Compare batch to batch. Consistency across lots is a stronger signal than any single certificate.
Research use only. This certificate describes material supplied strictly for laboratory research. It is not a drug, food, cosmetic or medical device, has not been evaluated by the FDA, and is not intended for human or veterinary administration of any kind.