Certificate of analysis
Semaglutide
Lot CP-SEMAGLE3-2607A · 5 mg
99.35 %
HPLC purity
Spec ≥ 99.0 %
90.7 %
Net peptide content
By amino acid analysis
< 0.11
Endotoxin (EU/mg)
Spec < 5.0 EU/mg
Provenance
- Testing laboratory
- Aurum Bioanalytics
- Accreditation
- ISO/IEC 17025:2017
- Country of synthesis
- United States
- Manufactured
- Jul 5, 2026
- Release tested
- Jul 14, 2026 (2 months ago)
- Retest date
- Jul 4, 2028
- CAS number
- 910463-68-2
- Molecular formula
- C187H291N45O59
- Theoretical mass
- 4113.58 Da
- Observed mass (LC-MS)
- 4113.59 Da (Δ 0.01 Da)
Assay results
Every line the release panel ran for this lot, with the method, the acceptance criterion and the measured value — not a pass mark alone.
| Method | Parameter | Specification | Result | |
|---|---|---|---|---|
| RP-HPLC (UV 214 nm) | Purity (area %) | ≥ 99.0 % | 99.35 % | Pass |
| LC-MS (ESI+) | Identity — observed mass | 4113.58 ± 1.0 Da | 4113.59 Da | Pass |
| Amino acid analysis | Net peptide content | ≥ 80.0 % | 90.7 % | Pass |
| Karl Fischer | Water content | ≤ 8.0 % | 2.26 % | Pass |
| Ion chromatography | Residual TFA | ≤ 1.0 % | 0.15 % | Pass |
| LAL kinetic chromogenic | Bacterial endotoxin | < 5.0 EU/mg | < 0.11 EU/mg | Pass |
| USP <71> membrane filtration | Sterility | No growth | No growth | Pass |
| Visual / USP <790> | Appearance | White to off-white cake, free of visible particulates | Conforms | Pass |
Sequence
H-His-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys(AEEA-AEEA-γGlu-C18 diacid)-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg-Gly-Arg-Gly-OH
Handling for this lot
- Appearance
- White to off-white lyophilised cake
- Storage
- Store lyophilised at -20 °C protected from light. Reconstituted material at 2–8 °C.
- Reconstitution
- Add diluent slowly down the vial wall and swirl — do not shake. Foaming shears long-chain acylated peptides and depresses recovered concentration.
Other lots of Semaglutide
Compare batch to batch. Consistency across lots is a stronger signal than any single certificate.
Research use only. This certificate describes material supplied strictly for laboratory research. It is not a drug, food, cosmetic or medical device, has not been evaluated by the FDA, and is not intended for human or veterinary administration of any kind.