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Third-party HPLC + mass spec on every lot
Verified — all release criteria met
released

Certificate of analysis

NAD+

Lot CP-NADPLUS-2604B · 500 mg

99.56 %

HPLC purity

Spec ≥ 99.0 %

87.3 %

Net peptide content

By amino acid analysis

< 0.22

Endotoxin (EU/mg)

Spec < 5.0 EU/mg

Provenance

Testing laboratory
Janoshik Analytical
Accreditation
ISO/IEC 17025:2017
Country of synthesis
United States
Manufactured
Apr 21, 2026
Release tested
Apr 30, 2026 (5 months ago)
Retest date
Apr 20, 2028
CAS number
53-84-9
Molecular formula
C21H27N7O14P2
Theoretical mass
663.43 Da
Observed mass (LC-MS)
663.50 Da (Δ 0.07 Da)

Assay results

Every line the release panel ran for this lot, with the method, the acceptance criterion and the measured value — not a pass mark alone.

Certificate of analysis — assay results
MethodParameterSpecificationResult
RP-HPLC (UV 214 nm)Purity (area %)≥ 99.0 %99.56 %Pass
LC-MS (ESI+)Identity — observed mass663.43 ± 1.0 Da663.50 DaPass
Amino acid analysisNet peptide content≥ 80.0 %87.3 %Pass
Karl FischerWater content≤ 8.0 %2.23 %Pass
Ion chromatographyResidual TFA≤ 1.0 %0.13 %Pass
LAL kinetic chromogenicBacterial endotoxin< 5.0 EU/mg< 0.22 EU/mgPass
USP <71> membrane filtrationSterilityNo growthNo growthPass
Visual / USP <790>AppearanceWhite to off-white cake, free of visible particulatesConformsPass
RP-HPLCResidual nicotinamide (hydrolysis product)≤ 0.5 %0.14 %Pass

Handling for this lot

Appearance
White to pale yellow lyophilised powder
Storage
-20 °C, desiccated. Hygroscopic — reseal immediately after opening.
Reconstitution
Prepare fresh. Aqueous NAD+ hydrolyses measurably within days even refrigerated, and the hydrolysis product inhibits sirtuins.

Other lots of NAD+

Compare batch to batch. Consistency across lots is a stronger signal than any single certificate.

Research use only. This certificate describes material supplied strictly for laboratory research. It is not a drug, food, cosmetic or medical device, has not been evaluated by the FDA, and is not intended for human or veterinary administration of any kind.